Document Type : Original Article
Authors
1
Department of Medical Physiology, Damietta Faculty of Medicine, Al-Azhar University, Damietta, Egypt.
2
Department of Medical Physiology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
Abstract
Background: A naturally occurring poison, arsenic can be found in food, soil, and water. Drinking water tainted with arsenic is the main way that people are exposed to the metal. Obeticholic acid (OCA) has been studied in a variety of conditions, its ability to treat hepatic damage is yet unknown. Natural antioxidants such total phenolics, ascorbic acid oxidase, polyphenol oxidase, catalase, and antioxidant vitamins A, C, and E may be found in moringa leaves. Essential amino acids including methionine, cysteine, tryptophan, lysine, as well as vitamins and minerals, are abundant in the leaves.
Objective: This study aims to discover how the administration of obeticholic acid and Moringa oleifera leaf extract affects the hepatotoxicity caused by arsenic in adult male albino rats.
Materials and Methods: To serve as the model for this study, fifty adult male albino rats of the local strain were selected.. Rats were randomly divided into 5 equal groups (10 rats each) as follows: Group I (control), Group II (Hepatotoxicity), Group III (Hepatotoxicity Plus obeticholic acid treated), Group IV (Hepatotoxicity Plus Moringaoleifera leaf extract treated) and Group V (Hepatotoxicity Plus obeticholic acid and Moringaoleifera leaf extract treated). Rats were slaughtered at the end of the experiment, and blood samples were obtained to measure high density lipoprotein (HDL), low density lipoprotein (LDL), triglycerides (TG), total cholesterol, aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), and L-lactate dehydrogenase (LDH).Superoxide dismutase (SOD), glutathione (GSH), albumin, malondialdehyde (MDA), catalase (CAT), and tumour necrosis factor alpha (TNF α). Nuclear factor kappa (NF-kB) immunohistochemistry and liver histopathology analysis validated the findings.
Results : The administration of arsenic can cause significant increase in Cholesterol, TGs, LDL, AST, ALT, ALP, MDA, SOD, GSH, TNF α associated with significant decrease in HDL, LD, Albumin, CAT.
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